Your contact is Principal Scientist Dr. Sanjay Tiwari

Fig. 1 Detection of phosphorylated histone 2AX (H2AX) in response to DNA damage as a measure of drug genotoxicity. DNA damage foci are shown in green, cell nuclei stained with DAPI in blue.

The Leica THUNDER Imager 3D Tissue is designed for real-time 3D fluorescence imaging of thick tissue sections and spheroids. Blurred image information is eliminated from areas outside the focal plane through computational clearing, a new opto-digital method from Leica Microsystems. This allows for high resolution images of the finest structures, deep in the specimen.

Additional Features:

• The monochrome camera utilizes scientific Metal Oxide semiconductor (sCMOS) technology for fluorescent imaging with high-speed acquisition of dynamic processes.
• The CoolLED pE-4000 multi-line LED light source has 16 selectable LED wavelengths ranging from 365nm – 770nm.
• Emission filters conform to DAPI, GFP, Texas Red, Cy5 and Cy7
• 5x objective suitable for phase contrast and differential interference contrast to enhance contrast using polarized light. A 20x Apochromatic color correction allows for color correction for optimal colocalization of fluorescent signals. A 63x oil immersion lens maintains homogenous immersion for focusing deep into tissue.
  

Fig. 2 Visualization of cell cycle progression via fluorescent ubiquitination-based cell cycle Indicator (FUCCI) expressing Pancreatic cancer cell line Colo-357 (obtained from Dr Ulf Geisen, UKSH). Cells in G1 are marked red and cells in S/G2/M are marked as green. Upper row: Untreated cells; Lower row: Cells treated with 1mM FUBIS  (5-fluorodeoxyuridine conjugated to alendronate) for 36h. Accumulation of cells in the S/G2/M phases is observed. Images were taken at 20x magnification, scale bar = 50µm. 

Fig. 3 Determination of bone formation rate with MgGd implants via fluorescent bone histomorphometry analyses. In vivo injected calcein green and xylenol orange form chelates with calcium ions. At sites of mineralization the fluorescent label is deposited to new bone surface and when injected on different days, the rate of bone formation can be measured. Left: 5x magnification, scale bar = 1mm; Right: 20x magnification, scale bar = 0.2mm.